作者单位
1 Department of Medical Ultrasound, The Second Affiliated Hospital, School of Medicine,
South China University of Technology, Guangzhou, China;
2 Department of Gastrointestinal Surgery, The Second Clinical Medical College,
Jinan University, Shenzhen China;
3 Department of Gastrointestinal Surgery, The First Affiliated Hospital,
Southern University of Science and Technology), Shenzhen, China
4 Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China;
5 SXUltrasonic (Shenzhen) Ltd., Shenzhen 518000, China;
6 Center for Cell and Gene Circuit Design, CAS Key Laboratory of Quantitative Engineering Biology,
Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology,
Chinese Academy of Sciences, Shenzhen, China
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内容概况
The epithelial-mesenchymal transition (EMT), a process in which epithelial cells undergo
a series of biochemical changes to acquire a mesenchymal phenotype, has been linked to tumor
metastasis. Here, we present a novel strategy for knocking out the EMT-related Cdh2 gene, which
encodes N-cadherin through CRISPR/Cas9-mediated gene editing by an ultrasound combined with
biosynthetic nanobubbles (Gas Vesicles, GVs). Polyethyleneimine were employed as a gene delivery
vector to deliver sgRNA into 4T1 cells that stably express the Cas9 protein, resulting in the stable
Cdh2 gene- knockout cell lines. TheWestern blotting assay confirmed the absence of an N-cadherin
protein in these Cdh2 gene-knockout 4T1 cell lines. Significantly reduced tumor cell migration was
observed in the Cdh2 gene-knockout 4T1 cells in comparison with the wild-type cells. Our study
demonstrated that an ultrasound combined with GVs could effectively mediate CRISPR/Cas9 gene
editing of a Cdh2 gene to inhibit tumor invasion and metastasis.